Experimental validation

The Experimental validation steps for the design and validation of molecular assays typically consist of:

  • the cloning of specific target zones into plasmids,
  • the design selection,
  • a specificity study against related species/genes,
  • the optimization of the experimental design,
  • the experimental characterisation and definition of the LOD (Limit Of Detection) and the LOQ (Limit Of Quantification),
  • the determination of the robustness of the assay,
  • the validation on biological samples.

Additional information